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The occurrence of three Ag NORs per metaphase
2021-05-25
The occurrence of three Ag-NORs per metaphase and/or a maximum of three nucleoli per nucleus in most of the varieties may be explained by chromosomal rearrangements or unequal crossing-over events that induce the deletion of the fourth active rDNA loci. Also, the chromosome pair that showed less int
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The methods for DNA MTase activity assay involve
2021-05-25
The methods for DNA MTase activity assay involve radioactive labelling [15], gel electrophoresis [16], high-performance liquid chromatography [17], [18], bisulfite conversion [19], [20], and affinity enrichment [21], [22]. In these methods, the methylated bases under the action of active MTase could
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The ribosomal synthesis of D proteins is
2021-05-25
The ribosomal synthesis of D-proteins is not currently feasible; the best effort undertaken so far was limited to the translational incorporation of two D-amino acids into the nascent protein chain by means of modified ribosomes (Dedkova et al., 2006). We therefore apply solid-phase peptide synthesi
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br Experimental procedures br Introduction Protein
2021-05-25
Experimental procedures Introduction Protein kinases represent one of the largest enzyme Z-VDVAD-FMK australia in the human genome and act as signaling mediators in a variety of cellular processes (Manning et al., 2002). Because many diseases are associated with aberrant protein kinase activit
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br Subjects and methods br Results br Discussion In the
2021-05-25
Subjects and methods Results Discussion In the present study, we found significantly increased CSF levels of IL-4, IL-5, IL-9, IL-10, TNF-α, CXCL8/IL-8, and VEGF-A together with significantly higher detection rates of IL-6, GM-CSF, PDGF-BB and IL-1ra in IHP patients compared with OND patien
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br Methods and materials br Cyclooxygenase
2021-05-25
Methods and materials Cyclooxygenase (COX) is the key enzyme required for the conversion of arachidonic bradykinin receptor antagonist to prostaglandin, prostacyclins and thromboxanes., , , Two distinct isoforms of COX were initially discovered: COX-1 is a constitutive, housekeeping enzyme th
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The proposed FDIR technique is presented
2021-05-25
The proposed FDIR technique is presented in the following format: Section 2 contains a survey of typical orbits of interest with regard to the Compstatin environment found in them, which is contrasted with a LEO orbit. Additionally, how the requirements for the satellite system can be estimated is p
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br Third trimester crossing the threshold to immunocompetenc
2021-05-25
Third trimester – crossing the threshold to immunocompetence Summary Regrettably, studies on the fetal immune system have yet to yield practical results. While it is becoming apparent that the fetal immune response plays a role in determining the outcome of congenital CMV infection, we still l
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SAR 405 Determination of relative potency EC by dose respons
2021-05-25
Determination of relative potency (EC50) by dose response assays in vitro is subject to the same sources of variability as determination of affinity, since the EC50 and Hill slope of the dose response curve are highly dependent on the binding of ligand to a competing receptor that may be present, an
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Next we elucidated that the Mtb enolase binds to
2021-05-24
Next, we elucidated that the Mtb enolase binds to host plasminogen with a high affinity. The KD value of 360nM for this interaction suggested an avid binding between the two proteins in vivo. This observation is also in conciliation with the observed KD values for enolase-plasminogen interactions in
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In regards to regional variation in
2021-05-24
In regards to regional variation in metabolic behavior of adipose tissue, subcutaneous adipose tissue transplantation has been shown to reprogram visceral adipose tissue to have subcutaneous-like phenotypic behavior, whereas visceral-to-subcutaneous transplantation does not promote a more detrimenta
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The monomeric oligomeric states of
2021-05-24
The monomeric/oligomeric states of DDR2-V5-His and DDR2-Fc were confirmed by Western blotting under reducing and non-reducing conditions. As shown in Fig. 1b, DDR2-V5-His exhibited a relative molecular mass of ~60 kDa under both reducing and non-reducing conditions, consistent with this protein bein
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In the phase I AURA trial patients with EGFR activating
2021-05-24
In the phase I AURA trial, patients with EGFR activating mutations and centrally confirmed tumor and/or plasma genotyping (BEAMing) T790M result were enrolled. Among 216 patients with both plasma and tissue genotyping results, the concordance rate was 82% for propane synthesis 19 deletions, 86% for
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br E E backside interaction The E possesses an
2021-05-24
E3–E2 backside interaction The E2 possesses an important regulatory interface which is termed its backside as it is opposite to the catalytic cleft that bears the active-site cysteine forming the thioester with SUMOD. This backside site interacts noncovalently with a scaffold SUMOB and was origin
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The relatively less well studied
2021-05-24
The relatively less well-studied encodes the E2 type SUMO-conjugating enzyme. In plants, the SCE family members play roles in abiotic stress responses. An E2 enzyme, SaSce9, from plays roles in salinity and drought stress responses (). Furthermore, SCE can respond to heat stress in rice (). Howeve
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