• How to Measure Cancer Drug Responses In Vitro

  2026-09-03

  Hannah R. Schwartz’s dissertation distinguishes relative viability from fractional viability, showing that growth inhibition and cell death are related but non-equivalent dimensions of anticancer drug response. This framework supports better assay design, endpoint selection, and interpretation of time-dependent drug effects in cancer biology and mechanistic inhibitor studies.

• Gut Microbiota, RPE/Choroid, and AMD Pathobiology

  2026-09-02

  The 2022 reference study connects the absence of gut microbiota with RPE/choroid transcriptomic changes, smaller laser-induced choroidal neovascularization lesions, and reduced microglial infiltration in mice. Its combined RNA-sequencing and ocular angiogenesis model provides evidence for a gut–RPE/choroidal axis while also illustrating the RNA-to-cDNA workflow demands of transcriptomic research.

• CD8+ T Cell Metabolic Flexibility via CD28–ARS2

  2026-09-02

  The reference study identifies a CD28–ARS2 signaling axis that reshapes PKM alternative splicing and enables activated CD8+ T cells to adjust glucose catabolism. Its findings separate this splicing mechanism from canonical CD28–PI3K signaling and connect PKM2 enrichment with interferon-γ production and antitumor effector activity.

• Protein A/G Magnetic Beads: Practical Guide

  2026-09-01

  Protein A/G Magnetic Beads (K1305) provide Fc-directed capture of IgG antibodies for antibody purification, immunoprecipitation, co-IP, and Ch-IP workflows from complex biological samples. They should be used as research-use affinity particles, with antibody compatibility, bead loading, wash conditions, and elution conditions optimized for each assay rather than assumed from a universal protocol.

• Phosbind Biotin LC: PVDF Workflow Guide

  2026-09-01

  Phosbind Biotin LC is a phosphate-binding reagent for sequence-independent detection of phosphorylated proteins on PVDF membranes using streptavidin-HRP and chemiluminescence. It is suited to Western Blot phosphorylated protein detection, but not to aqueous-only workflows, long-term storage of prepared solutions, or residue-specific phosphorylation assignments.

• Pifithrin-α (PFTα): A p53 Inhibitor Guide

  2026-08-31

  Pifithrin-α is a p53 inhibitor used to test p53-dependent apoptosis, growth arrest, and ferroptosis mechanisms. This guide connects product handling with evidence from a maternal deltamethrin neurotoxicity study while defining preclinical limits.

• NADH: Reduced Nicotinamide Adenine Dinucleotide

  2026-08-31

  NADH, or reduced nicotinamide adenine dinucleotide, is an electron-carrying coenzyme that connects glycolytic and tricarboxylic-acid-cycle metabolism with mitochondrial ATP production. Its research value depends on compartment, redox context, assay conditions, and careful separation of NADH effects from NAD+-dependent signaling.

• SETD7 Depletion Promotes White Fat Browning

  2026-08-30

  This study identifies SETD7 as a negative regulator of inguinal white adipose tissue thermogenesis and links its depletion to an Adcy7–Sirt1–CREB1 signaling axis. The findings provide a mechanistic framework for studying adipose browning and metabolic protection in obese mice, while also highlighting important limits for translation to human metabolic disease.

• Medroxyprogesterone Acetate in Decidualization Assays

  2026-08-29

  Explore how Medroxyprogesterone acetate and MPA can be used as receptor-aware perturbations in endometrial decidualization research. This guide translates vitamin D/VDR findings into practical assay decisions while clearly separating validated evidence from exploratory applications.

• Bradykinin B2 Receptors and Ileal Peristalsis

  2026-08-28

  Chan and Rudd showed that bradykinin suppresses the peristaltic reflex in the isolated guinea pig ileum through pharmacologically defined B2 receptors, rather than B1 receptors. The study establishes a quantitative ex vivo framework for linking kinin receptor signaling to intestinal propulsion and clarifies how agonist, antagonist, and comparator responses can be interpreted.

• CRISPR/Cas9 mRNA–gRNA Editing of LGMN

  2026-08-28

  This study developed a lipid nanoparticle strategy for co-delivering Cas9 mRNA and guide RNA to disrupt LGMN, a lysosomal protease associated with aggressive breast cancer behavior. The work links an in vitro transcription-based RNA production workflow with reduced lysosomal/autophagic degradation, migration, invasion, and experimental lung metastasis, while also highlighting the preclinical limitations of this approach.

• Cy5-UTP: Direct Fluorescent RNA Labeling

  2026-08-27

  Cy5-UTP, also called Cyanine 5-uridine triphosphate, is a fluorescent UTP analog for T7 RNA polymerase-mediated in vitro transcription RNA labeling. It enables direct visualization of labeled RNA at excitation and emission maxima of 650 and 670 nm without a separate staining step, subject to assay-specific incorporation and detection controls.

• SEMA3E, Beige Fat, and β-Catenin Thermogenesis

  2026-08-27

  The 2026 reference study identifies SEMA3E as a regulator of beige adipocyte differentiation and cold-induced thermogenesis in mice. Through genetic perturbation, adipose transplantation, mitochondrial respiration assays, RNA sequencing, and pathway rescue experiments, it links SEMA3E activity to controlled Wnt/β-catenin signaling and oxidative phosphorylation.

• HyperScribe Co-transcription mRNA Kit Plus: Assay Guide

  2026-08-26

  This scenario-based guide explains how capped, polyadenylated mRNA design affects cell viability, proliferation, cytotoxicity, and translation experiments. It shows where HyperScribe™ Co-transcription mRNA Synthesis Kit Plus (ARCA, T7), SKU K1406, can improve workflow standardization while distinguishing product-supported facts from laboratory recommendations.

• Bestatin Inhibition of Leucine Aminopeptidase

  2026-08-26

  The 1991 PNAS study used high-resolution X-ray crystallography to show how Bestatin, also known as Ubenimex, occupies the catalytic site of bovine lens leucine aminopeptidase. Its zinc-coordinating groups and substrate-like side-chain contacts support a structural model in which Bestatin resembles a tetrahedral intermediate, providing a mechanistic framework for interpreting aminopeptidase inhibition and related biochemical assays.

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